Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
$226.92
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Product Details
General description
Amicon® Ultra-2 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at 100% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
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Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
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