Blunt/TA Ligase Master Mix will ligate these substrates:

dsDNA

Nicked DNA/RNA

 

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.

• Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components
• No thawing is necessary as it remains liquid during storage at -20°C
• Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
• Suitable for Adaptor Ligation
• Compatible with several Oxford Nanopore Technologies^® workflows, including some involving Covid-19 research
• Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4 and Instant Sticky-end Ligase Master Mix
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart