DraI - 2,000 units - R0129S

DraI - 2,000 units - R0129S

• Product Information

  Product Source

  An E. coli strain that carries the DraI gene from Deinococcus radiophilus (ATCC 27603).

  This product is related to the following categories:

   

  Restriction Endonucleases C G Products, 

  Time-Saver Qualified Restriction Enzymes Products

  This product can be used in the following applications: 

  Fast Cloning: Accelerate your cloning workflows with reagents from NEB, 

  Restriction Enzyme Digestion

  • Reagents Supplied

    The following reagents are supplied with this product:

    NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
    R0129S     -20       DraI R0129SVIAL -20 1 x 0.1 ml 20,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
    R0129L     -20       DraI R0129LVIAL -20 1 x 0.5 ml 20,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

  • Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

    Reaction Conditions

    1X rCutSmart™ Buffer
    Incubate at 37°C

    1X rCutSmart™ Buffer
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    100 µg/ml Recombinant Albumin
    (pH 7.9 @ 25°C)

    Activity in NEBuffers

    NEBuffer™ r1.1: 75%
    NEBuffer™ r2.1: 75%
    NEBuffer™ r3.1: 50%
    rCutSmart™ Buffer: 100%
    

    Diluent Compatibility

    • Diluent A

    Storage Buffer

    10 mM Tris-HCl
    50 mM KCl
    1 mM DTT
    0.1 mM EDTA
    200 µg/ml Recombinant Albumin
    50% Glycerol
    pH 7.4 @ 25°C

    Heat Inactivation

    65°C for 20 minutes

    Methylation Sensitivity

    dam methylation: Not Sensitive
    dcm methylation: Not Sensitive
    CpG Methylation: Not Sensitive

    Isoschizomers

    AhaIII+

  • Related Products

    Companion Products

    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    Materials Sold Separately

    • rCutSmart™ Buffer

  • Product Notes

    1. DraI is an isoschizomer of AhaIII.
    2. Not sensitive to CpG, dcm, or dam methylation.