E. coliDNA Ligase catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates.E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.E. coliDNA Ligase is active at a range of temperatures (4° C – 37° C).
Highlights
Specific Activity: 6,000 units/mg
Supplied with 10X Reaction Buffer
Product Source
Purified from E. coli strain containing a clonedE. coliDNA Ligase gene.
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