EHA105 Agrobacterium Chemically Competent Cells - 5x50uL - CC-108-5x50

EHA105 Agrobacterium Chemically Competent Cells - 5x50uL - CC-108-5x50

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Kit Components

• Competent Cells
• 1 x 12 mL Recovery Media
• 1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL)

Product Specifications

Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 10⁴ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

Storage/Handling: This product may be shipped on dry ice. EHA105 Agrobacterium chemically competent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

Reagents Needed for One Reaction

• EHA105 Chemical Competent Agrobacterium: 50 µL
• DNA (pCAMBIA1391z Control, 10 ng/µL): 5 µL
• Recovery medium: 1 mL

Table 1: Antibiotic disc sensitivity for GoldBio’s Agrobacterium strains (using standard BD antibiotic discs)