IBA LifeSciences Strep-TactinXT 4Flow high capacity column; 1 ml - 50-210-0160/25032001
Strep-TactinXT 4Flow high capacity columns contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein.