Indole-3-butyric acid (IBA) has been used as a standard to monitor IBA in urine samples by tandem mass spectrometry-selected reaction monitoring (SRM) and Ultra-high performance liquid chromatography (UHPLC)[1], as a standard for high performance liquid chromatography (HPLC) for the quantification of IBA in foliar tissues ofCoffea canephoraseedlings[2].
Biochem/physiol Actions
Indole-3-butyric acid (IBA) is a naturally occurring phytohormone auxin (plant growth regulator). It promotes root formation in cuttings but does not affect ethylene levels. IBA might be a precursor for indole-3-acetic acid (IAA) through β oxidation pathway. IBA is present in plant species likeZea mays,Pisum sativumandArabidopsis. IBA is more potent than IAA for inducing root formation.[3]