Western Blot was performed using Anti-ARF6 Polyclonal Antibody (Product # PA1-093) and a 25 kDa band corresponding to ARF6 was observed across cell lines with uncharacterized band(*). Whole cell extracts (30 µg lysate) of LNCaP (Lane 1), MDA-MB-231 (Lane 2), HUVEC (Lane 3), MCF7 (Lane 4), MCF 10A (Lane 5), SK-BR-3 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System