Invitrogen F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot™ 605 - 200uL - Q-11001MP
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Invitrogen F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot™ 605 - 200uL - Q-11001MP
Mouse IgG (H+L) Secondary Antibody (Q-11001MP) in WB
Multiplexed fluorescent western blot was performed using F(ab)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot™ 605 (Product # Q-11001MP). Whole cell extracts of A-431 (Lane 1, 2), HaCaT (Lane 3, 4, 5), SH-SY5Y (Lane 6), HeLa (Lane 7) and MCF7 (Lane 8) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB23001) byiBlot® 2 Dry BlottingSystem (Product # IB21001). The blot was probed with Cytokeratin 5 Monoclonal Antibody (3E2F1) (Product # MA5-15347), and HSP70 Polyclonal Antibody (Product # PA5-28003). Secondary antibodies (Product # Q-11001MP, 1:500 dilution), and (Product # A32808, 1:20000 dilution) were used for detection of Cytokeratin 5, and HSP70 respectively. Fluorescent detection was performed usingiBrightFL1500 (Product # A44115). The anti-mouse secondary antibody (Product # R37115) specifically detects the mouse primary antibody.
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Invitrogen F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot™ 605 - 200uL - Q-11001MP
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