Monarch® RNase A - 1ml - T3018L

Name: Monarch® RNase A - 1ml - T3018L
Brand: NEB
SKU: T3018L
Price: 78.08 USD
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Product Details

Monarch® RNase A - 1ml - T3018L

Product Information

Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types. Monarch RNase A degrades single-stranded RNA at C and U residues. This formulation is purified from cow pancreas, is free of contaminating protease or DNase activity, and contains no residual host DNA. It is used at 56°C in the Monarch Genomic DNA Purification Kit protocol but is active at any temperature between 20°C and 65°C. It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4.

Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low; however, an RNase A treatment step is included in each protocol as an option for the user.

This product is related to the following categories:

RNases,

Genomic DNA Extraction & Purification Products,

Nucleic Acid Purification Products

This product can be used in the following applications:: Nucleic Acid Purification

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

T3018L

-20

Monarch^® RNase A T3018-2 -20 2 x 0.5 ml 20 mg/ml

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Monarch® RNase A - 1ml - T3018L