BL21(DE3) Competent E. coli
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BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain. Size 20 x 0.05 ml
Ideal for routine T7 expression
Protease deficient B strain
No dry ice surcharge on competent cell shipments
Outgrowth medium included
Free of animal products
Transformation Efficiency
1–5 x 107 cfu/µg pUC19 DNA
Genotype
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration
C2527H -80
SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable
pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
BL21(DE3) Competent E. coli C2527HVIAL -80 20 x 0.05 ml Not Applicable