NEB® 10-beta Competent E. coli (High Efficiency) - 20 x 0.05ml - C3019H
$252.78
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Product Details
NEB® 10-beta Competent E. coli (High Efficiency) - 20 x 0.05ml - C3019H
Product Information
Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.
Highlights
- DH10B™ derivative
- Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA)
- Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
- Reduced recombination of cloned DNA (recA1)
- Clone large plasmids and BACs
- Free of animal products
- K12 Strain
Transformation Efficiency
For C3019I and C3019H: 1–3 x 109 cfu/µg pUC19 DNAFor C3019P: 1–3 x 108 cfu/µg pUC19 DNA
Genotype
Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)- This product is related to the following categories:
- This product can be used in the following applications:
- USER® Cloning,
- Applications of USER® and Thermolabile USER II Enzymes,
- Site Directed Mutagenesis,
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NEB® 10-beta Competent E. coli (High Efficiency) - 20 x 0.05ml - C3019H
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