PpuMI - 500 units - R0506S

PpuMI - 500 units - R0506S

$76.13

Pre-order

Out of stock, available for pre-order

Quantity:

1

Product Details

PpuMI - 500 units - R0506S

Product Information

Product Source

An E. coli strain that carries the PpuMI gene from Pseudomonas putida (R. Morgan).

This product is related to the following categories:

Restriction Endonucleases P R Products,

Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

	PpuMI	R0506SVIAL	-20	1 x 0.05 ml	10,000 units/ml
	rCutSmart™ Buffer	B6004SVIAL	-20	1 x 1.25 ml	10 X

	PpuMI	R0506LVIAL	-20	1 x 0.25 ml	10,000 units/ml
	rCutSmart™ Buffer	B6004SVIAL	-20	1 x 1.25 ml	10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: <10%
NEBuffer™ r2.1: <10%
NEBuffer™ r3.1: <10%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent B
Storage Buffer

10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Blocked by Overlapping
CpG Methylation: Not Sensitive

Isoschizomers

Psp5II
PspPPI

Save this product for later

PpuMI - 500 units - R0506S