Promocell GmbH PromoCell Endothelial Cell Growth Medium 2 500 ml, ready-to-use, low serum, with Supplement Mix C-39216 attached to the flask Supplier: Promocell GmbH C22011 500ml Catalog:50-306-189

Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its multiple effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.