Protein A Magnetic Beads - 1ml - S1425S
$203.01
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Product Details
Protein A Magnetic Beads - 1ml - S1425S
Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein A that is covalently coupled to a nonporous superparamagnetic particle.
- Exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse
- The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants
- The matrix can be regenerated without loss of binding capacity
- Can be used to immunoprecipitate target proteins from crude cell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target antigen
- Binding capacity: 1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG
- Quick, small-scale purification of many subclasses of IgGs affords higher throughput and efficiency
Product Information
Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. A truncated form of recombinant Protein A is covalently coupled to a nonporous superparamagnetic particle. Protein A exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can then be regenerated without loss of binding capacity. Protein A Magnetic Beads can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.
- This product is related to the following categories:
- This product can be used in the following applications:
- ChIP,
- DNA Enrichment,
- Antibodies,
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Reagents Supplied
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Properties & Usage
Storage Buffer
0.05% NaN3
0.1% BSA
0.05% Tween® 20
1X PBS
pH 7.4 @ 25°CSupport Matrix
2 µm nonporous superparamagnetic microparticlesBinding Capacity
1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG.Can be Regenerated
Yes -
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Protein A Magnetic Beads - 1ml - S1425S
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