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Protein A Magnetic Beads - 1ml - S1425S

Protein A Magnetic Beads - 1ml - S1425S

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Product Details

Protein A Magnetic Beads - 1ml - S1425S

Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein A that is covalently coupled to a nonporous superparamagnetic particle. 

  • Exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse
  • The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants 
  • The matrix can be regenerated without loss of binding capacity
  • Can be used to immunoprecipitate target proteins from crude cell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target antigen 
  • Binding capacity: 1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG
  • Quick, small-scale purification of many subclasses of IgGs affords higher throughput and efficiency

Product Information

Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. A truncated form of recombinant Protein A is covalently coupled to a nonporous superparamagnetic particle. Protein A exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can then be regenerated without loss of binding capacity. Protein A Magnetic Beads can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3).

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.


This product is related to the following categories:
 
Anitibodies Products, 
Magnetic Beads and Racks Products
This product can be used in the following applications: 
ChIP, 
DNA Enrichment, 
Antibodies,
 Pull Down Assays, 5-hmC Detection & Analysis, High-throughput cloning and automation solutions, Phage Display
  • Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    • S1425S     4    
        Protein A Magnetic Beads S1425SVIAL 4 1 x 1 ml Not Applicable
  • Properties & Usage

    Storage Buffer

    0.05% NaN3
    0.1% BSA
    0.05% Tween® 20
    1X PBS
    pH 7.4 @ 25°C

    Support Matrix

    2 µm nonporous superparamagnetic microparticles

    Binding Capacity

    1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG.

    Can be Regenerated

    Yes
  • Related Products

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    • Protein G Magnetic Beads
    • Streptavidin Magnetic Beads
    • 50 ml Magnetic Separation Rack
    • Chitin Magnetic Beads
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