Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

• Highest fidelity amplification (~280X higher than Taq)
• Master mix includes Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg⁺⁺ in a broad-use buffer
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and hot start master mix formats (NEB #M0494)