Ribonucleotide Solution Mix - 50umol - N0466L

Ribonucleotide Solution Mix - 50umol - N0466L

• Buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP
• Supplied as sodium salts, pH 7.5
• Also available as Ribonucleotide Solution Set (NEB #N0450)
• Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.

• Product Information

  Ribonucleotide Solution Mix is a buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP, pH 7.5 as sodium salts. The NTPs are also available as Ribonucleotide Solution Set (NEB #N0450).
  
  Supplied as: Sodium salts, pH 7.5
  
  Reagents Supplied (small):
  NTP Mix (25 mM of each)   0.4 ml
  
  Reagents Supplied (large): 
  NTP Mix (25 mM of each)   5 x 0.4 ml
  
  Diluent Compatibility:
  Can be diluted using nuclease-free water

  This product is related to the following categories:

   

  Nucleotide Solutions Products, 

  RNA Synthesis In vitro Transcription (IVT)

  This product can be used in the following applications: 

  Transcription-Mediated and NASBA Amplification, 

  PCR

  • Reagents Supplied

    The following reagents are supplied with this product:

    NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
    N0466S     -20       Ribonucleotide Solution Mix N0466SVIAL -20 1 x 0.4 ml 25 mM
    N0466L     -20       Ribonucleotide Solution Mix N0466SVIAL -20 5 x 0.4 ml 25 mM

  • Advantages and Features

    Application Features

    • In vitro transcription

  • Related Products

    Companion Products

    • SP6 RNA Polymerase
    • T7 RNA Polymerase
    • HiScribe^®; T7 High Yield RNA Synthesis Kit

  • Product Notes

    1. Store at -20°C. Avoid multiple freeze/thawcycles. Dilute with nuclease-free water whennecessary.