LGSAVLQ-Rh110-dP is a fluorogenic substrate for measuring the 3CL protease activity of coronaviruses. The sequence is derived from the SARS CoV-2 3CLpro. Release of Rhodamine 110 can be monitored with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Conditions will need to be optimized for each specific application. We recommend an initial substrate concentration of 4-20 μM.
