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Taq DNA Ligase - 10,000 units - M0208L

Taq DNA Ligase - 10,000 units - M0208L

$351.36
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Product Details

Taq DNA Ligase - 10,000 units - M0208L

Taq DNA Ligase has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10233580.

Try HiFi Taq DNA Ligase, for superior fidelity and thermostability

 

Taq DNA Ligase will ligate these substrates:

Nicked DNA/RNA





  • Isolated from a recombinant source
  • Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
  • Taq DNA Ligase is NOT a substitute for T4 DNA Ligase
  • Supplied with 10X Reaction Buffer containing NAD+
  • Free Thermostable Ligase ReactionTemperature Calculator tool to estimate appropriate incubation temperature
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

Product Information

Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).  

Product Source

Purified from an E. coli strain containing the cloned ligase gene from Thermus thermophilus HB8 (1-3).
This product is related to the following categories:
 
DNA Ligases Products
This product can be used in the following applications: 
Cloning Ligation, 
Gibson Assembly®
  • Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    • M0208S     -20    
        Taq DNA Ligase M0208SVIAL -20 1 x 0.05 ml 40,000 units/ml
        Taq DNA Ligase Reaction Buffer B0208SVIAL -20 1 x 1.5 ml 10 X
    • M0208L     -20    
        Taq DNA Ligase M0208LVIAL -20 1 x 0.25 ml 40,000 units/ml
        Taq DNA Ligase Reaction Buffer B0208SVIAL -20 1 x 1.5 ml 10 X
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